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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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ANR27 rabbit pAb
- 货号:YT7774
- 应用:WB
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human ANR27 AA range: 206-256
- 特异性:
- This antibody detects endogenous levels of ANR27 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- function:May be a Rab21 guanine exchange factor and regulate endosome dynamics.,similarity:Contains 1 VPS9 domain.,similarity:Contains 11 ANK repeats.,subcellular location:Mainly localized to early endosomes in the perinuclear region.,subunit:Interacts with RAB21 and RAB5, with a strong preference for RAB21.,
- 细胞定位:
- Early endosome . Late endosome . Cytoplasmic vesicle membrane . Lysosome . Cell membrane . Melanosome . Colocalizes with VAMP7 in transport vesicles in the shaft of hippocampal neurons (By similarity). .
- Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night