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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GPAT4 rabbit pAb
- 货号:YT7454
- 应用:WB
- 种属:Human;Mouse
- 简介:
- >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways
- 基因名称:
- AGPAT6 GPAT4 UNQ551/PRO1108
- 免疫原:
- Synthesized peptide derived from human GPAT4 AA range: 39-89
- 特异性:
- This antibody detects endogenous levels of GPAT4 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Lysophosphatidic acid acyltransferases (EC 2.3.1.51) catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA). LPA and PA are involved in signal transduction and lipid biosynthesis.[supplied by OMIM, Apr 2004],
- 功能:
- catalytic activity:Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.,domain:The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.,enzyme regulation:Inhibited by N-ethylmaleimide (NEM).,function:Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Active against both saturated and unsaturated long-chain fatty acyl-CoAs.,pathway:Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.,similarity:Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.,tissue specificity:Ubiquitous. Relatively high level of expression in skeletal muscle, heart and testis. Relatively low level of expression in lung.,
- 细胞定位:
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- 组织表达:
- Ubiquitous. High levels in testis. Relatively high level of expression in skeletal muscle and heart. Relatively low level of expression in lung.
![](/uploadfiles/PIC/YT7454wb-2019.jpg)
- Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°over night