RIFK rabbit pAb

    • 货号:YT7290
    • 应用:WB;ELISA;IHC
    • 种属:Human;Mouse
      • 靶点:
      • RIFK
      • 简介:
      • >>Riboflavin metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors
      • 基因名称:
      • RFK
      • 蛋白名称:
      • RIFK
      • Human Swiss Prot No:
      • Q969G6
      • Mouse Swiss Prot No:
      • Q8CFV9
      • 免疫原:
      • Synthesized peptide derived from human RIFK AA range: 82-132
      • 特异性:
      • This antibody detects endogenous levels of RIFK at Human/Mouse
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 分子量:
      • 17kD
      • 背景:
      • Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009],
      • 功能:
      • catalytic activity:ATP + riboflavin = ADP + FMN.,cofactor:Zinc or magnesium.,function:Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).,pathway:Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.,subunit:Monomer.,tissue specificity:Detected in brain, placenta and urinary bladder.,
      • 细胞定位:
      • Cytoplasm .
      • 组织表达:
      • Detected in brain, placenta and urinary bladder.
      • 产品图片
      • Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night
      • Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).