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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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AFAF rabbit pAb
- 货号:YT7165
- 应用:WB
- 种属:Human;Rat;Mouse;
- 免疫原:
- Synthesized peptide derived from human AFAF AA range: 223-273
- 特异性:
- This antibody detects endogenous levels of AFAF at Human
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- function:Involved in acrosome biogenesis.,tissue specificity:Isoform 1 highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.,
- 细胞定位:
- Cytoplasmic vesicle, secretory vesicle, acrosome membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, secretory vesicle, acrosome inner membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, secretory vesicle, acrosome outer membrane ; Single-pass type I membrane protein . In the anterior acrosome region, enriched on the inner acrosomal membrane but minimal on the outer acrosomal membrane; in contrast in the posterior acrosome region enriched on both the inner and outer acrosomal membranes. .
- 组织表达:
- Isoform 1 is highly expressed in testis. Isoform 2 is expressed at low levels in skin and blood.
![](/uploadfiles/PIC/YT7165wb-2019.jpg)
- Western blot analysis of lysates from HEK293 cells, primary antibody was diluted at 1:1000, 4°over night