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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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NIT1 rabbit pAb
- 货号:YT7119
- 应用:WB
- 种属:Human;Mouse;Rat
- 免疫原:
- Synthesized peptide derived from human NIT1 AA range: 32-82
- 特异性:
- This antibody detects endogenous levels of NIT1 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],
- 功能:
- sequence caution:Chimeric cDNA. Its C-terminal exon is derived from the last exon of the DEDD gene.,similarity:Belongs to the UPF0012 family.,similarity:Contains 1 CN hydrolase domain.,tissue specificity:Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.,
- 细胞定位:
- [Isoform 2]: Mitochondrion .; [Isoform 1]: Cytoplasm .
- 组织表达:
- Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
![](/uploadfiles/PIC/YT7119wb-2019.jpg)
- Western blot analysis of lysates from PC-12 cells, primary antibody was diluted at 1:1000, 4°over night