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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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TRAD1 rabbit pAb
- 货号:YT7113
- 应用:WB
- 种属:Human;Mouse;Rat
- 免疫原:
- Synthesized peptide derived from human TRAD1 AA range: 52-102
- 特异性:
- This antibody detects endogenous levels of TRAD1 at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses (Sanada et al., 2008 [PubMed 18849341]).[supplied by OMIM, Dec 2009],
- 功能:
- function:May function as a lipopolysaccharide (LPS)-inducible negative regulator in innate immunity. May be involved in endotoxin tolerance. Seems to act as a negative regulator in toll-like receptor signaling.,induction:LPS, IFNB1 and IFNG increased protein levels in RAW cells with IFNB1 being most potent inducer. IL4 and IL10 did not induced the expression in RAW cells, moreover, LPS did not induce expression in the presence of IL4 or IL10.,similarity:Contains 1 TRAF-type zinc finger.,subunit:Interacts with TRAF6.,
![](/uploadfiles/PIC/YT7113wb-2019.jpg)
- Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°over night