• 靶点：
• DHE3

• 简介：
• >>Arginine biosynthesis;>>Alanine, aspartate and glutamate metabolism;>>Nitrogen metabolism;>>Metabolic pathways;>>Carbon metabolism;>>Necroptosis;>>Proximal tubule bicarbonate reclamation

• 基因名称：
• GLUD1 GLUD

• 蛋白名称：
• DHE3

• Human Gene Id：
• 2746

• Human Swiss Prot No：
• P00367

• Mouse Gene Id：
• 14661

• Mouse Swiss Prot No：
• P26443

• Rat Gene Id：
• 24399

• Rat Swiss Prot No：
• P10860

• 免疫原：
• Synthesized peptide derived from human DHE3

• 特异性：
• This antibody detects endogenous levels of DHE3 at Human/Mouse/Rat

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1：500-2000

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 分子量：
• 61kD

• 背景：
• This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X. [provided by RefSeq, Jan 2016],

• 功能：
• catalytic activity:L-glutamate + H(2)O + NAD(P)(+) = 2-oxoglutarate + NH(3) + NAD(P)H.,disease:Defects in GLUD1 are the cause of hyperinsulinism-hyperammonemia syndrome (HHS) [MIM:606762]. Elevated oxidation rate of glutamate to alpha-ketoglutarate stimulates insulin secretion in the pancreatic beta cells, while they impair detoxification of ammonium in the liver.,enzyme regulation:Subject to allosteric regulation. Activated by ADP. Inhibited by GTP and ATP. ADP can occupy the NADH binding site and activate the enzyme.,function:May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.,online information:Glutamate dehydrogenase 1 entry,similarity:Belongs to the Glu/Leu/Phe/Val dehydrogenases family.,subunit:Homohexamer.,

• 细胞定位：
• Mitochondrion . Endoplasmic reticulum . Mostly translocates into the mitochondria, only a small amount of the protein localizes to the endoplasmic reticulum. .

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