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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PMM1 rabbit pAb
- 货号:YT7034
- 应用:WB;IHC
- 种属:Human;Mouse
- 简介:
- >>Fructose and mannose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Metabolic pathways;>>Biosynthesis of cofactors;>>Biosynthesis of nucleotide sugars
- 免疫原:
- Synthesized peptide derived from human PMM1 AA range: 18-68
- 特异性:
- This antibody detects endogenous levels of PMM1 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate.,function:Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.,pathway:Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.,similarity:Belongs to the eukaryotic PMM family.,tissue specificity:Strong expression in liver, heart, brain, and pancreas; lower expression in skeletal muscle.,
- 组织表达:
- Strong expression in liver, heart, brain, and pancreas; lower expression in skeletal muscle.
![](/uploadfiles/PIC/YT7034wb-2019.jpg)
- Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/42zhongfenhuaaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).