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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DET1 rabbit pAb
- 货号:YT6984
- 应用:WB
- 种属:Human;Mouse
- 简介:
- >>Ubiquitin mediated proteolysis
- 免疫原:
- Synthesized peptide derived from human DET1 AA range: 48-98
- 特异性:
- This antibody detects endogenous levels of DET1 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- function:Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.,similarity:Belongs to the DET1 family.,subunit:Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1.,tissue specificity:Highly expressed in the ovary, some lymphoid organs and resting leukocytes.,
- 组织表达:
- Highly expressed in the ovary, some lymphoid organs and resting leukocytes.
![](/uploadfiles/PIC/YT6984wb-2019.jpg)
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night