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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SC22C rabbit pAb
- 货号:YT6946
- 应用:WB;IF;ELISA;IHC
- 种属:Human;Mouse
- 基因名称:
- SEC22C SEC22L3 UNQ459/PRO784
- 免疫原:
- Synthesized peptide derived from human SC22C AA range: 130-180
- 特异性:
- This antibody detects endogenous levels of SC22C at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000; IF ICC 1:50-200;ELISA 1:2000-20000;IHC 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a member of the SEC22 family of vesicle trafficking proteins. The encoded protein is localized to the endoplasmic reticulum and may play a role in the early stages of ER-Golgi protein trafficking. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011],
- 功能:
- function:May be involved in vesicle transport between the ER and the Golgi complex.,similarity:Belongs to the synaptobrevin family.,similarity:Contains 1 longin domain.,tissue specificity:Ubiquitously expressed.,
- 细胞定位:
- Endoplasmic reticulum membrane ; Multi-pass membrane protein .
- 组织表达:
- Ubiquitously expressed.
![](/uploadfiles/PIC/YT6946wb-2019.jpg)
- Western blot analysis of lysates from MDA-MB cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/66ganaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).