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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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T2FA rabbit pAb
- 货号:YT6890
- 应用:WB
- 种属:Human;Mouse;Rat
- 简介:
- >>Basal transcription factors
- 免疫原:
- Synthesized peptide derived from human T2FA AA range: 462-512
- 特异性:
- This antibody detects endogenous levels of T2FA at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- caution:Was reported (PubMed:10428810) to have a protein kinase activity and to autophosphorylates on Ser-385 and Thr-389.,function:TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.,PTM:Phosphorylated on Ser and other residues by TAF1 and casein kinase II-like kinases.,similarity:Belongs to the TFIIF alpha subunit family.,subunit:Heterodimer of an alpha and a beta subunit. Interacts with GTF2F2 and CTDP1. Interacts with TAF6/TAFII80.,
- Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°over night