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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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VP37C rabbit pAb
- 货号:YT6677
- 应用:WB
- 种属:Human;Mouse
- 免疫原:
- Synthesized peptide derived from human VP37C AA range: 220-270
- 特异性:
- This antibody detects endogenous levels of VP37C at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]).[supplied by OMIM, Mar 2008],
- 功能:
- function:Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation.,similarity:Belongs to the VPS37 family.,similarity:Contains 1 VPS37 C-terminal domain.,subcellular location:Probably associates with membranes. Recruited to the plasma membrane by HIV-1.,subunit:Component of the ESCRT-I complex (endosomal sorting complex required for transport I) which consists of TSG101, VPS28, a VPS37 protein (VPS37A to -D) and a FAM125/MVB12 protein (FAM125A or -B) in a 1:1:1:1 stoechiometry. Interacts with TSG101, VPS28, FAM125A and FAM125B. Interacts with HGS and STAM2. Interacts with CEP55.,
- 细胞定位:
- Late endosome membrane ; Peripheral membrane protein . Probably associates with membranes. Recruited to the plasma membrane by HIV-1.
![](/uploadfiles/PIC/YT6677wb-2019.jpg)
- Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°over night