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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GLDN rabbit pAb
- 货号:YT6545
- 应用:WB
- 种属:Human;Mouse;Rat
- 基因名称:
- GLDN COLM UNQ9339/PRO34011
- 免疫原:
- Synthesized peptide derived from human GLDN AA range: 461-511
- 特异性:
- This antibody detects endogenous levels of GLDN at Human/Mouse/Rat
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 功能:
- domain:The olfactomedin-like domain mediates NFASC/neurofascin and NRCAM binding.,function:Plays a role in the formation of the nodes of Ranvier along myelinated axons. Probable NRCAM and NFASC/neurofascin ligand which may provide a glial positional clue required for the proper molecular assembly of the nodes of Ranvier.,similarity:Contains 1 olfactomedin-like domain.,similarity:Contains 2 collagen-like domains.,subcellular location:Localizes to the nodes of Ranvier.,subunit:Interacts with NFASC/neurofascin and NRCAM.,tissue specificity:Specifically expressed in spinal cord, brain, placenta and sciatic nerve. More abundant in peripheral than central nervous system.,
- 细胞定位:
- Cell membrane ; Single-pass type II membrane protein . Cell projection, axon . Detected at the nodes of Ranvier. Detected at immature heminodes. .; [Gliomedin shedded ectodomain]: Secreted . Secreted, extracellular space, extracellular matrix . Proteolytic processing gives rise to a soluble extracellular domain that is secreted. The gliomedin shedded ectodomain localizes to the nodes of Ranvier. .
- 组织表达:
- Specifically expressed in spinal cord, brain, placenta and sciatic nerve. More abundant in peripheral than central nervous system.
![](/uploadfiles/PIC/YT6545wb-2019.jpg)
- Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night