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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PURA1 rabbit pAb
- 货号:YT6536
- 应用:WB;IHC
- 种属:Human;Mouse
- 简介:
- >>Purine metabolism;>>Alanine, aspartate and glutamate metabolism;>>Metabolic pathways;>>Nucleotide metabolism;>>Biosynthesis of cofactors
- 免疫原:
- Synthesized peptide derived from human PURA1 AA range: 14-64
- 特异性:
- This antibody detects endogenous levels of PURA1 at Human/Mouse
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- This gene encodes a member of the adenylosuccinate synthase family of proteins. The encoded muscle-specific enzyme plays a role in the purine nucleotide cycle by catalyzing the first step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP). Mutations in this gene may cause adolescent onset distal myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],
- 功能:
- catalytic activity:GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.,cofactor:Binds 1 magnesium ion per subunit.,function:Plays an important role in the de novo pathway of purine nucleotide biosynthesis.,pathway:Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.,similarity:Belongs to the adenylosuccinate synthetase family.,subunit:Homodimer.,tissue specificity:Predominantly expressed in skeletal muscle and heart, as well as in several hematopoietic cell lines and solid tumors.,
- 组织表达:
- Predominantly expressed in skeletal muscle and heart, as well as in several hematopoietic cell lines and solid tumors.
![](/uploadfiles/PIC/YT6536wb-2019.jpg)
- Western blot analysis of lysates from VEC cells, primary antibody was diluted at 1:1000, 4°over night
![](/uploadfiles/PIC/48ganaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).