GRASP Polyclonal Antibody
- 货号：YT6213
- 应用：IHC;IF;WB
- 种属：Human;Mouse;Rat
- 免疫原：
- Synthesized peptide derived from human GRASP
- 特异性：
- This antibody detects endogenous levels of human GRASP
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:50-200, WB 1:500-2000. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- General receptor for phosphoinositides 1-associated scaffold protein (GRP1-associated scaffold protein)
- 背景：
- This gene encodes a protein that functions as a molecular scaffold, linking receptors, including group 1 metabotropic glutamate receptors, to neuronal proteins. The encoded protein contains conserved domains, including a leucine zipper sequence, PDZ domain and a C-terminal PDZ-binding motif. Alternately spliced transcript variants have been observed for this gene.[provided by RefSeq, Dec 2012],
- 功能：
- function:Plays a role in intracellular trafficking and contributes to the macromolecular organization of group 1 metabotropic glutamate receptors (mGluRs) at synapses.,similarity:Contains 1 PDZ (DHR) domain.,subcellular location:Localized at postsynaptic membranes.,subunit:Heteromer. Composed of GRASP, PSCD2 and at least one GRM1. Also interacts with PSCD3, GRM2, GRM3 and GRM5.,
- 细胞定位：
- Cytoplasm, perinuclear region . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse, postsynaptic cell membrane .
- Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
