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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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TIG1 Polyclonal Antibody
- 货号:YT6186
- 应用:IHC;IF;WB
- 种属:Human
- 免疫原:
- Synthesized peptide derived from human TIG1
- 特异性:
- This antibody detects endogenous levels of human TIG1
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:50-200, WB 1:500-2000. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Retinoic acid receptor responder protein 1 (RAR-responsive protein TIG1;Tazarotene-induced gene 1 protein)
- 背景:
- This gene was identified as a retinoid acid (RA) receptor-responsive gene. It encodes a type 1 membrane protein. The expression of this gene is upregulated by tazarotene as well as by retinoic acid receptors. The expression of this gene is found to be downregulated in prostate cancer, which is caused by the methylation of its promoter and CpG island. Alternatively spliced transcript variant encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
- 功能:
- induction:By tazarotene and by all the retinoic acid receptors tested.,similarity:Belongs to the protease inhibitor I47 (latexin) family.,
- 细胞定位:
- Membrane ; Single-pass type III membrane protein .
![](/uploadfiles/PIC/YN2874wb-2020.jpg)
- Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night