HLA-DMβ Polyclonal Antibody
- 货号：YT6025
- 应用：IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Phagosome;>>Cell adhesion molecules;>>Antigen processing and presentation;>>Hematopoietic cell lineage;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>Intestinal immune network for IgA production;>>Type I diabetes mellitus;>>Leishmaniasis;>>Toxoplasmosis;>>Staphylococcus aureus infection;>>Tuberculosis;>>Influenza A;>>Human T-cell leukemia virus 1 infection;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Asthma;>>Autoimmune thyroid disease;>>Inflammatory bowel disease;>>Systemic lupus erythematosus;>>Rheumatoid arthritis;>>Allograft rejection;>>Graft-versus-host disease;>>Viral myocarditis
- 蛋白名称：
- HLA class II histocompatibility antigen, DM beta chain (MHC class II antigen DMB) (Really interesting new gene 7 protein)
- 免疫原：
- Synthetic peptide from human protein at AA range: 40-100
- 特异性：
- The antibody detects endogenous HLA-DMβ
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:50-200, ELISA 1:10000-20000. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- HLA class II histocompatibility antigen, DM beta chain (MHC class II antigen DMB;Really interesting new gene 7 protein)
- 背景：
- HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008],
- 功能：
- domain:The YXXZ (Tyr-Xaa-Xaa-Zaa, where Zaa is a hydrophobic residue) motif mediates the targeting to the lysosomal compartments.,function:Plays a critical role in catalyzing the release of class II HLA-associated invariant chain-derived peptides (CLIP) from newly synthesized class II HLA molecules and freeing the peptide binding site for acquisition of antigenic peptides.,polymorphism:The following alleles of DMB are known: DMB*0101, DMB*0102, DMB*0103, DMB*0104 (DMB3.4), DMB*0105 and DMB*0106. The sequence shown is that of DMB*0101.,similarity:Belongs to the MHC class II family.,similarity:Contains 1 Ig-like C1-type (immunoglobulin-like) domain.,subcellular location:Localizes to late endocytic compartment. Associates with lysosome membranes.,subunit:Heterodimer of an alpha chain (DMA) and a beta chain (DMB).,
- 细胞定位：
- Late endosome membrane ; Single-pass type I membrane protein . Lysosome membrane ; Single-pass type I membrane protein . Localizes to late endocytic compartment. Associates with lysosome membranes.
- 组织表达：
- Blood,Lymph,Plasma,Spleen,
- Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded Human-colon, antibody was diluted at 1:100
