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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MIA Polyclonal Antibody
- 货号:YT6015
- 应用:IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Melanoma-derived growth regulatory protein (Melanoma inhibitory activity protein)
- 免疫原:
- Synthetic peptide from human protein at AA range: 90-131
- 特异性:
- The antibody detects endogenous MIA
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC 1:50-200, ELISA 1:10000-20000. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Melanoma-derived growth regulatory protein (Melanoma inhibitory activity protein)
- 背景:
- function:Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.,PTM:May possess two intramolecular disulfide bonds.,similarity:Belongs to the MIA/OTOR family.,similarity:Contains 1 SH3 domain.,tissue specificity:All malignant melanoma cell lines tested and infrequently in glioma cell lines.,
- 功能:
- function:Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.,PTM:May possess two intramolecular disulfide bonds.,similarity:Belongs to the MIA/OTOR family.,similarity:Contains 1 SH3 domain.,tissue specificity:All malignant melanoma cell lines tested and infrequently in glioma cell lines.,
- 组织表达:
- All malignant melanoma cell lines tested and infrequently in glioma cell lines.
- Immunohistochemical analysis of paraffin-embedded Human-heart, antibody was diluted at 1:100