- 靶点:
- Beclin 1
- 简介:
- >>Autophagy - other;>>Mitophagy - animal;>>Autophagy - animal;>>Apoptosis - multiple species;>>Apelin signaling pathway;>>Alzheimer disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Spinocerebellar ataxia;>>Pathways of neurodegeneration - multiple diseases;>>Shigellosis;>>Kaposi sarcoma-associated herpesvirus infection
- 基因名称:
- BECN1 GT197
- 蛋白名称:
- Beclin-1
- Human Gene Id:
- 8678
- Human Swiss Prot No:
- Q14457
- Mouse Gene Id:
- 56208
- Mouse Swiss Prot No:
- O88597
- 免疫原:
- Synthesized peptide derived from Beclin-1 . at AA range: 110-190
- 特异性:
- Beclin-1 Polyclonal Antibody detects endogenous levels of Beclin-1
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000, ELISA 1:10000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- beclin 1, autophagy related
- 实测条带:
- 55kD
- 背景:
- beclin 1(BECN1) Homo sapiens This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
- 功能:
- function:Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.,similarity:Belongs to the beclin family.,subcellular location:Expressed in dendrites and cell bodies of cerebellar Purkinje cells.,subunit:Interacts with GOPC and GRID2. Interacts with AMBRA1. Probably forms a complex with AMBRA1 and PIK3C3 (By similarity). Interacts with BCL2 and BCL2L1.,tissue specificity:Ubiquitous.,
- 细胞定位:
- Cytoplasm . Golgi apparatus, trans-Golgi network membrane ; Peripheral membrane protein . Endosome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein . Mitochondrion membrane ; Peripheral membrane protein . Endosome . Cytoplasmic vesicle, autophagosome . Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity). .; [Beclin-1-C 35 kDa]: Mitochondrion . Nucleus . Cytoplasm .; [Beclin-1-C 37 kDa]: Mitochondrion .
- 组织表达:
- Ubiquitous.
α-Lipoic acid improves abnormal behavior by mitigation of oxidative stress, inflammation, ferroptosis, and tauopathy in P301S Tau transgenic mice. Redox Biology 2017 Nov 02 WB Mouse 1:1000 brain
货号:YT5763
Ammonia inhalation-mediated mir-202-5p leads to cardiac autophagy through PTEN/AKT/mTOR pathway. CHEMOSPHERE Chemosphere. 2019 Nov;235:858 WB Chicken 1:1000 heart
货号:YT5763
Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB Mouse liver
货号:YT5763
miR-2954 Inhibits PI3K Signaling and Induces Autophagy and Apoptosis in Myocardium Selenium Deficiency. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Nov 21 WB Chicken 1:500 cardiomyocytes
货号:YT5763
Mitophagy is activated in brain damage induced by cerebral ischemia and reperfusion via the PINK1/Parkin/p62 signalling pathway. BRAIN RESEARCH BULLETIN Brain Res Bull. 2018 Sep;142:63 WB Rat 1:1000 cortex
货号:YT5763
Fasting Therapy Contributes to the Improvement of Endothelial Function and Decline in Vascular Injury-Related Markers in Overweight and Obese Individuals via Activating Autophagy of Endothelial Progenitor Cells. Evidence-based Complementary and Alternative Medicine 2020 Jul 27 WB Mouse 1 : 1000 Endothelial progenitor cells
货号:YT5763
Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy. Frontiers in Immunology 2018 Aug 16 WB Mouse liver
货号:YT5763
The natural medicinal fungus Huaier promotes the anti-hepatoma efficacy of sorafenib through the mammalian target of rapamycin-mediated autophagic cell death MEDICAL ONCOLOGY Zhengguang Zhang WB Mouse, Human
货号:YT5763
Nobiletin Alleviated Epithelial–Mesenchymal Transition of Hepatocytes in Liver Fibrosis Based on Autophagy—Hippo/YAP Pathway MOLECULAR NUTRITION & FOOD RESEARCH Yuting Hao WB Human L02 cell
货号:YT5763
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- Antibody-FAQs
- 产品图片
- Zhang, Yan-Hui, et al. "α-Lipoic acid improves abnormal behavior by mitigation of oxidative stress, inflammation, ferroptosis, and tauopathy in P301S Tau transgenic mice." Redox biology 14 (2018): 535-548.
- Western blot analysis of lysates from 1) mouse-brain , 2) mouse-lung, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(2B8) (cat:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.
- Western Blot analysis of mouse-brain mouse-lung cells using Beclin-1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Feng, Qin. "Quantitative proteomic analysis reveals that Arctigenin alleviates concanavalin A-induced hepatitis through suppressing immune system and regulating autophagy." Frontiers in immunology 9 (2018): 1881.