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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CD72 Polyclonal Antibody
- 货号:YT5709
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 简介:
- >>B cell receptor signaling pathway
- 蛋白名称:
- B-cell differentiation antigen CD72
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the Internal region of human CD72. AA range:170-220
- 特异性:
- CD72 Polyclonal Antibody detects endogenous levels of CD72 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- IHC: 100-300.WB 1:500 - 1:2000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CD72;B-cell differentiation antigen CD72;Lyb-2;CD72
- 背景:
- function:Plays a role in B-cell proliferation and differentiation. Associates with CD5.,online information:CD72,similarity:Contains 1 C-type lectin domain.,subunit:Homodimer; disulfide-linked.,tissue specificity:Pre-B-cells and B-cells but not terminally differentiated plasma cells.,
- 功能:
- function:Plays a role in B-cell proliferation and differentiation. Associates with CD5.,online information:CD72,similarity:Contains 1 C-type lectin domain.,subunit:Homodimer; disulfide-linked.,tissue specificity:Pre-B-cells and B-cells but not terminally differentiated plasma cells.,
- 细胞定位:
- Membrane; Single-pass type II membrane protein.
- 组织表达:
- Pre-B-cells and B-cells but not terminally differentiated plasma cells.
- Western blot analysis of RAT-SPLEEN MOUSE-BRAIN MOUSE-SPLEEN lysis using CD72 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded Human spleen. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).