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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CD56 Polyclonal Antibody
- 货号:YT5708
- 应用:WB;IF;ELISA
- 种属:Human
- 简介:
- >>Cell adhesion molecules;>>Prion disease
- 蛋白名称:
- Neural cell adhesion molecule 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the Internal region of human NCAM1. AA range:250-300
- 特异性:
- CD56 Polyclonal Antibody detects endogenous levels of CD56 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000;ELISA: 1:10000;IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- NCAM1;NCAM;Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56
- 背景:
- This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011],
- 功能:
- function:This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.,online information:N-CAM 140,online information:NCAM entry,similarity:Contains 2 fibronectin type-III domains.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains.,
- 细胞定位:
- [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 4]: Cell membrane ; Lipid-anchor, GPI-anchor .; [Isoform 5]: Secreted.; [Isoform 6]: Secreted .
- 组织表达:
- Brain,Brain cortex,Kidney,Ovary,Plasma,Skeletal muscle,Testis,
- Western blot analysis of mouse-lung mouse-heart lysis using NCAM1 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200
- Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
- Western Blot analysis of Hela cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000