Lefty Polyclonal Antibody
- 货号:YT5647
- 应用:WB;IHC
- 种属:Human;Mouse
- 简介:
- >>TGF-beta signaling pathway;>>Signaling pathways regulating pluripotency of stem cells
- 蛋白名称:
- Left-right determination factor 1/Left-right determination factor 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the C-terminal region of human LEFTY1/LEFTY2. AA range:301-350
- 特异性:
- Lefty Polyclonal Antibody detects endogenous levels of Lefty protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- LEFTY1;LEFTB;LEFTYB;Left-right determination factor 1;Left-right determination factor B;Protein lefty-1;Protein lefty-B;LEFTY2;EBAF;LEFTA;LEFTYA;TGFB4;PSEC0024;Left-right determination factor 2;Endometrial bleeding-associated factor;Left-right determination factor A;Protein lefty-2;Protein lefty-A;Transforming growth factor beta-4;TGF-beta-4
- 背景:
- This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene. [provided by RefSeq, Aug 2016],
- 功能:
- function:Required for left-right axis determination as a regulator of LEFTY2 and NODAL.,PTM:The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.,similarity:Belongs to the TGF-beta family.,
- 组织表达:
- Colon,Pancreas,Spleen,Teratocarcinoma,
![](/uploadfiles/PIC/yt5647-wb.jpg)
- Western Blot analysis of HepG2-UV cells using Lefty Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
![](/uploadfiles/PIC/yt5647-wb(2).jpg)
- Western Blot analysis of HEPG2-UV cells using Lefty Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
![](/uploadfiles/PIC/215feilinaicc.jpg)
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).