Bag-3 Polyclonal Antibody
- 货号：YT5544
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- BAG family molecular chaperone regulator 3
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the Internal region of human BAG3. AA range:311-360
- 特异性：
- Bag-3 Polyclonal Antibody detects endogenous levels of Bag-3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- BAG3;BIS;BAG family molecular chaperone regulator 3;BAG-3;Bcl-2-associated athanogene 3;Bcl-2-binding protein Bis;Docking protein CAIR-1
- 背景：
- BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008],
- 功能：
- function:Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.,similarity:Contains 1 BAG domain.,similarity:Contains 2 WW domains.,subunit:Binds to the ATPase domain of HSP70/HSC chaperones. Binds to Bcl-2 and PLC-gamma.,
- 细胞定位：
- Nucleus . Cytoplasm . Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920). .
- 组织表达：
- Brain,Epithelium,Liver,Lung,Placenta,T-cell,Testis,Tongue,
- Western Blot analysis of A549 cells using Bag-3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
