• 靶点：
• NCoA-3

• 简介：
• >>Endocrine resistance;>>Estrogen signaling pathway;>>Thyroid hormone signaling pathway;>>Pathways in cancer;>>Breast cancer

• 基因名称：
• NCOA3

• 蛋白名称：
• Nuclear receptor coactivator 3

• Human Gene Id：
• 8202

• Human Swiss Prot No：
• Q9Y6Q9

• Mouse Swiss Prot No：
• O09000

• Rat Swiss Prot No：
• Q9EPU2

• 免疫原：
• Synthesized peptide derived from the Internal region of human NCoA-3.

• 特异性：
• NCoA-3 Polyclonal Antibody detects endogenous levels of NCoA-3 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• NCOA3;AIB1;BHLHE42;RAC3;TRAM1;Nuclear receptor coactivator 3;NCoA-3;ACTR;Amplified in breast cancer 1 protein;AIB-1;CBP-interacting protein;pCIP;Class E basic helix-loop-helix protein 42;bHLHe42;Receptor-associated coactivator 3;RAC-3;Steroid receptor coactivator protein 3;SRC-3;Thyroid hormone receptor activator molecule 1;TRAM-1

• 实测条带：
• 160kD

• 背景：
• The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010],

• 功能：
• alternative products:Additional isoforms seem to exist,catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,domain:Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).,enzyme regulation:Coactivator activity on nuclear receptors and NF-kappa-B pathways is enhanced by various hormones, and the TNF cytokine, respectively. TNF stimulation probably enhances phosphorylation, which in turn activates coactivator function. In contrast, acetylation by CREBBP apparently suppresses coactivation of target genes by disrupting its association with nuclear receptors.,function:Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubuni

• 细胞定位：
• Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.

• 组织表达：
• Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

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