• 靶点：
• HMGB1

• 简介：
• >>Base excision repair;>>Autophagy - animal;>>Necroptosis;>>Neutrophil extracellular trap formation

• 基因名称：
• HMGB1

• 蛋白名称：
• High mobility group protein B1

• Human Gene Id：
• 3146

• Human Swiss Prot No：
• P09429

• Mouse Gene Id：
• 100862258

• Mouse Swiss Prot No：
• P63158

• Rat Gene Id：
• 25459

• Rat Swiss Prot No：
• P63159

• 免疫原：
• Synthesized peptide derived from the N-terminal region of human HMG-1.

• 特异性：
• HMG-1 Polyclonal Antibody detects endogenous levels of HMG-1 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• IF 1:50-200 WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HMGB1;HMG1;High mobility group protein B1;High mobility group protein 1;HMG-1

• 实测条带：
• about 30kd

• 背景：
• This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015],

• 功能：
• function:Binds preferentially single-stranded DNA and unwinds double stranded DNA.,similarity:Belongs to the HMGB family.,similarity:Contains 2 HMG box DNA-binding domains.,

• 细胞定位：
• Nucleus . Chromosome . Cytoplasm . Secreted . Cell membrane ; Peripheral membrane protein ; Extracellular side . Endosome . Endoplasmic reticulum-Golgi intermediate compartment . In basal state predominantly nuclear. Shuttles between the cytoplasm and the nucleus (PubMed:12231511, PubMed:17114460). Translocates from the nucleus to the cytoplasm upon autophagy stimulation (PubMed:20819940). Release from macrophages in the extracellular milieu requires the activation of NLRC4 or NLRP3 inflammasomes (By similarity). Passively released to the extracellular milieu from necrotic cells by diffusion, involving the fully reduced HGMB1 which subsequently gets oxidized (PubMed:19811284). Also released from apoptotic cells (PubMed:16855214, PubMed:18631454). Active secretion from a variety of immune a

• 组织表达：
• Ubiquitous. Expressed in platelets (PubMed:11154118).

Huaier Induces Immunogenic Cell Death Via CircCLASP1/PKR/eIF2α Signaling Pathway in Triple Negative Breast Cancer WB Human /MDA-MB-231 cells
货号：YT5502

HMGB1 contributes to allergen-induced airway remodeling in a murine model of chronic asthma by modulating airway inflammation and activating lung fibroblasts. Cellular & Molecular Immunology Cell Mol Immunol. 2014 Aug;12(4):409-423 WB,IHC Mouse 1∶300 left lung
货号：YT5502

HMGB1 could restrict 1,3-β-glucan induced mice lung inflammation by affecting Beclin1 and Bcl2 interaction and promoting the autophagy of epithelial cells. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Oct;222:112460 IF Mouse Lung
货号：YT5502

Hui, Zhi, and Zhe Li. "Continuous veno-venous hemofiltration for septic shock." INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 10.10 (2017): 14983-14988.
货号：YT5502

HMGB1 could restrict 1, 3-β-glucan induced mice lung inflammation by affecting Beclin1 and Bcl2 interaction and promoting the autophagy of epithelial cells." Ecotoxicology and Environmental Safety 222 (2021): 112460.
货号：YT5502

The protective role of HMGB1 in affecting the balance between autophagy and pyroptosis to maintain neutrophils homeostasis during β-glucan-induced mice lung inflammation ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Fangwei Liu IF Mouse lung
货号：YT5502

Multifunctional Metal-coordinated Co-assembled Carrier-free Nanoplatform Based on Dual-drugs for Ferroptosis-mediated Cocktail Therapy of Hepatocellular Carcinoma Growth and Metastasis JOURNAL OF COLLOID AND INTERFACE SCIENCE Rui-Rui Zhao IF Human HepG2 cell
货号：YT5502

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