L-Selectin Polyclonal Antibody
- 货号：YT5370
- 应用：WB;ELISA;IHC
- 种属：Human;Mouse;Rat
- 简介：
- >>Cell adhesion molecules
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the Internal region of human SELL. AA range:91-140
- 特异性：
- L-Selectin Polyclonal Antibody detects endogenous levels of L-Selectin protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SELL;LNHR;LYAM1;L-selectin;CD62 antigen-like family member L;Leukocyte adhesion molecule 1;LAM-1;Leukocyte surface antigen Leu-8;Leukocyte-endothelial cell adhesion molecule 1;LECAM1;Lymph node homing receptor;TQ1;gp90-MEL;CD62L
- 背景：
- This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009],
- 功能：
- function:Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.,online information:L-selectin,similarity:Belongs to the selectin/LECAM family.,similarity:Contains 1 C-type lectin domain.,similarity:Contains 1 EGF-like domain.,similarity:Contains 2 Sushi (CCP/SCR) domains.,subunit:Interaction with PSGL1/SELPLG is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan and tyrosine sulfation modifications of PSGL1. Sulfation on Y-51 of PSGL1 is important for L-selectin binding.,tissue specificity:Expressed in B cell lines and T lymphocytes.,
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达：
- Expressed in B-cell lines and T-lymphocytes.
- Western Blot analysis of A549 cells using L-Selectin Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from A549 cells, using SELL Antibody.
- Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
