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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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VHR Polyclonal Antibody
- 货号:YT5228
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>MAPK signaling pathway
- 蛋白名称:
- Dual specificity protein phosphatase 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from the C-terminal region of human DUSP3. AA range:136-185
- 特异性:
- VHR Polyclonal Antibody detects endogenous levels of VHR protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DUSP3;VHR;Dual specificity protein phosphatase 3;Dual specificity protein phosphatase VHR;Vaccinia H1-related phosphatase;VHR
- 背景:
- The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast ca
- 功能:
- catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,catalytic activity:Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate.,function:This protein shows activity both toward tyrosine-protein phosphate as well as with serine-protein phosphate.,similarity:Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.,similarity:Contains 1 tyrosine-protein phosphatase domain.,
![](/uploadfiles/PIC/yt5228-wb.jpg)
- Western Blot analysis of HepG2 cells using VHR Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt5228ihcmouse-kidney.jpg)
- Immunohistochemical analysis of paraffin-embedded mouse-kidney, antibody was diluted at 1:100
![](/uploadfiles/PIC/yt5228_pic796318522.jpg)
- Western blot analysis of lysate from HepG2 cells, using DUSP3 Antibody.