c-FLIP Polyclonal Antibody
- 货号：YT5156
- 应用：WB;ELISA
- 种属：Human;Mouse
- 简介：
- >>NF-kappa B signaling pathway;>>Autophagy - animal;>>Apoptosis;>>Necroptosis;>>TNF signaling pathway;>>Chagas disease;>>Hepatitis C
- 蛋白名称：
- CASP8 and FADD-like apoptosis regulator
- 免疫原：
- The antiserum was produced against synthesized peptide derived from the N-terminal region of human CFLAR. AA range:1-50
- 特异性：
- c-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CFLAR;CASH;CASP8AP1;CLARP;MRIT;CASP8 and FADD-like apoptosis regulator;Caspase homolog;CASH;Caspase-eight-related protein;Casper;Caspase-like apoptosis regulatory protein;CLARP;Cellular FLICE-like inhibitory protein;c-FLIP;FADD-like antiapoptotic molecule 1;FLAME-1;Inhibitor of FLICE;I-FLICE;MACH-related inducer of toxicity;MRIT;Usurpin
- 背景：
- The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011],
- 功能：
- domain:The caspase domain lacks the active sites residues involved in catalysis.,function:Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.,induction:Repressed by IL-2 after TCR stimulation, during progression to the S-phase of the cell cycle.,PTM:Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.,similarity:Belongs to the peptidase C14A family.,similarity:Contains
- 细胞定位：
- cytoplasm,cytosol,death-inducing signaling complex,CD95 death-inducing signaling complex,membrane raft,ripoptosome,
- 组织表达：
- Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
- Western Blot analysis of SW480, SKOV3, HepG2 cells using c-FLIP Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from SW480 cells, using CFLAR Antibody.
