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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PREP Polyclonal Antibody
- 货号:YT5132
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Prolyl endopeptidase
- 免疫原:
- Synthesized peptide derived from PREP . at AA range: 10-90
- 特异性:
- PREP Polyclonal Antibody detects endogenous levels of PREP protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- PREP;PEP;Prolyl endopeptidase;PE;Post-proline cleaving enzyme
- 背景:
- The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.,function:Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.,PTM:The N-terminus is blocked.,similarity:Belongs to the peptidase S9A family.,subunit:Monomer.,
- 细胞定位:
- nucleus,cytoplasm,membrane,
- 组织表达:
- Blood,Brain,Lymphocyte,Skin,
- Western Blot analysis of Jurkat cells using PREP Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
- Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100