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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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RhCG Polyclonal Antibody
- 货号:YT5088
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Ammonium transporter Rh type C
- 免疫原:
- Synthesized peptide derived from the Internal region of human RhCG.
- 特异性:
- RhCG Polyclonal Antibody detects endogenous levels of RhCG protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- RHCG;C15orf6;CDRC2;PDRC2;RHGK;Ammonium transporter Rh type C;Rh glycoprotein kidney;Rhesus blood group family type C glycoprotein;Rh family type C glycoprotein;Rh type C glycoprotein;Tumor-related protein DRC2
- 背景:
- developmental stage:Specifically expressed in fetal kidney.,function:Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.,PTM:N-glycosylated.,similarity:Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.,subcellular location:Also detected at the basolateral membrane and in subapical vesicles.,tissue specificity:Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). Specifically expressed in kidney according to PubMed:11062476.,
- 功能:
- developmental stage:Specifically expressed in fetal kidney.,function:Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion.,PTM:N-glycosylated.,similarity:Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.,subcellular location:Also detected at the basolateral membrane and in subapical vesicles.,tissue specificity:Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). Specifically expressed in kidney according to PubMed:11062476.,
- 细胞定位:
- Apical cell membrane ; Multi-pass membrane protein . Also detected at the basolateral membrane and in subapical vesicles. .
- 组织表达:
- Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney.
- Western Blot analysis of extracts from Jurkat, K562 cells, using RhCG Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:100