UHRF1 Polyclonal Antibody
- 货号：YT5066
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- E3 ubiquitin-protein ligase UHRF1
- 免疫原：
- Synthesized peptide derived from the Internal region of human UHRF1.
- 特异性：
- UHRF1 Polyclonal Antibody detects endogenous levels of UHRF1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- UHRF1;ICBP90;NP95;RNF106;E3 ubiquitin-protein ligase UHRF1;Inverted CCAAT box-binding protein of 90 kDa;Nuclear protein 95;Nuclear zinc finger protein Np95;HuNp95;hNp95;RING finger protein 106;Transcription factor ICBP90;Ubiquitin-like PHD and RING finger domain-containing protein 1;hUHRF1;Ubiquitin-like-containing PHD and RING finger domains protein 1
- 背景：
- This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014],
- 功能：
- developmental stage:Expressed in fetal thymus, liver and kidney.,domain:The RING finger is required for ubiquitin ligase activity.,domain:The YDG domain mediates the interaction with histone H3.,function:Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.,induction:Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a TP53/p53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity.,PTM:Ubiquitinated; which leads to proteasomal degrad
- 细胞定位：
- Nucleus . Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
- 组织表达：
- Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.
- Western Blot analysis of extracts from Jurkat cells, using UHRF1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
