AR-β2 Polyclonal Antibody
- 货号:YT5048
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- Adrenergic Receptor β2
- 简介:
- >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Adrenergic signaling in cardiomyocytes;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Salivary secretion;>>Chemical carcinogenesis - receptor activation
- 蛋白名称:
- Beta-2 adrenergic receptor
- 免疫原:
- Synthesized peptide derived from AR-β2 . at AA range: 300-380
- 特异性:
- AR-β2 Polyclonal Antibody detects endogenous levels of AR-β2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ADRB2;ADRB2R;B2AR;Beta-2 adrenergic receptor;Beta-2 adrenoreceptor;Beta-2 adrenoceptor
- 背景:
- This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
- 功能:
- disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the recep
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). .
- 组织表达:
- Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid,
- Western Blot analysis of extracts from K562 cells, using AR-β2 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000