α4Gn-T Polyclonal Antibody
- 货号：YT5000
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- Alpha-1,4-N-acetylglucosaminyltransferase
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human A4GNT. AA range:31-80
- 特异性：
- α4Gn-T Polyclonal Antibody detects endogenous levels of α4Gn-T protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- A4GNT;Alpha-1;4-N-acetylglucosaminyltransferase;Alpha4GnT
- 背景：
- This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008],
- 功能：
- domain:The conserved DXD motif is involved in enzyme activity.,function:Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.,online information:Alpha-1,4-N-acetylglucosaminyltransferase,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 32 family.,tissue specificity:Detected in stomach and pancreas.,
- 细胞定位：
- Golgi apparatus membrane ; Single-pass type II membrane protein .
- 组织表达：
- Detected in stomach and pancreas.
- Western blot analysis of lysates from K562 cells, using A4GNT Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
