ZnT-8 Polyclonal Antibody
- 货号：YT4990
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human SLC30A8. AA range:171-220
- 特异性：
- ZnT-8 Polyclonal Antibody detects endogenous levels of ZnT-8 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- SLC30A8;ZNT8;Zinc transporter 8;ZnT-8;Solute carrier family 30 member 8
- 背景：
- The protein encoded by this gene is a zinc efflux transporter involved in the accumulation of zinc in intracellular vesicles. This gene is expressed at a high level only in the pancreas, particularly in islets of Langerhans. The encoded protein colocalizes with insulin in the secretory pathway granules of the insulin-secreting INS-1 cells. Allelic variants of this gene exist that confer susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010],
- 功能：
- domain:Contains a histidine-rich region, HXXXXXHNH-motif, which is a ligand for zinc.,function:Facilitates the accumulation of zinc from the cytoplasm into intracellular vesicles, being a zinc-efflux transporter. May be a major component for providing zinc to insulin maturation and/or storage processes in insulin-secreting pancreatic beta-cells.,polymorphism:Variant Trp-325 is a risk factor that confers susceptibility to diabetes mellitus, noninsulin-dependent (NIDDM) [MIM:125853].,similarity:Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.,subcellular location:Strictly associated with the insulin granule secretory.,tissue specificity:Strictly restricted to the pancreas islets cells. Found in insulin-producing beta cells and colocalized with insulin.,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle, secretory vesicle membrane ; Multi-pass membrane protein . Associated with insulin and glucagon secretory granules. .
- 组织表达：
- In the endocrine pancreas, expressed in insulin-producing beta cells. Expressed at relatively high levels in subcutaneous fat tissue from lean persons; much lower levels in visceral fat, whether from lean or obese individuals, and in subcutaneous fat tissue from obese individuals. Expressed in peripheral blood mononuclear cells, including T-cells and B-cells, with great variation among individuals ranging from negative to strongly positive.
- Western Blot analysis of K562 cells using ZnT-8 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysates from K562 cells, using SLC30A8 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using SLC30A8 antibody.
