VPS54 Polyclonal Antibody
- 货号：YT4890
- 应用：IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Vacuolar protein sorting-associated protein 54
- 免疫原：
- Synthesized peptide derived from the Internal region of human VPS54.
- 特异性：
- VPS54 Polyclonal Antibody detects endogenous levels of VPS54 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- VPS54;HCC8;Vacuolar protein sorting-associated protein 54;Hepatocellular carcinoma protein 8;Tumor antigen HOM-HCC-8;Tumor antigen SLP-8p
- 背景：
- This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008],
- 功能：
- function:May be involved in retrograde transport from early and late endosomes to late Golgi.,similarity:Belongs to the VPS54 family.,subunit:Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS52, VPS53 and VPS54.,
- 细胞定位：
- Golgi apparatus, trans-Golgi network . Membrane . Associates with membranes in an EIPR1-independent manner. .
- 组织表达：
- Hepatoma,Placenta,Skin,Testis,
- Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
