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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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VIP Polyclonal Antibody
- 货号:YT4883
- 应用:IHC;IF;WB;ELISA
- 种属:Human;Rat;Mouse;
- 简介:
- >>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human VIP. AA range:71-120
- 特异性:
- VIP Polyclonal Antibody detects endogenous levels of VIP protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014],
- 功能:
- function:PHM and PHV also cause vasodilation.,function:VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.,online information:Vasoactive intestinal peptide entry,similarity:Belongs to the glucagon family.,
- 组织表达:
- Pancreatic carcinoma,Pheochromocytoma,Prostate,
- Immunohistochemical analysis of paraffin-embedded Human testis. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Immunohistochemistry analysis of VIP antibody in paraffin-embedded human testis tissue.
- Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night