• 靶点：
• Vinculin

• 简介：
• >>Focal adhesion;>>Adherens junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Amoebiasis

• 基因名称：
• VCL

• 蛋白名称：
• Vinculin

• Human Gene Id：
• 7414

• Human Swiss Prot No：
• P18206

• Mouse Gene Id：
• 22330

• Mouse Swiss Prot No：
• Q64727

• Rat Gene Id：
• 305679

• Rat Swiss Prot No：
• P85972

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Vinculin. AA range:786-835

• 特异性：
• Vinculin Polyclonal Antibody detects endogenous levels of Vinculin protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• VCL;Vinculin;Metavinculin

• 实测条带：
• 123kD

• 背景：
• Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],

• 功能：
• disease:Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.,online information:Vinculin entry,PTM:Aceylated; mainly by myristic acid but also small amount of palmitic acid.,PTM:Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.,similarity:Belongs to the vinculin/alpha-catenin family.,s

• 细胞定位：
• Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .

• 组织表达：
• Metavinculin is muscle-specific.

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