V-ATPase H Polyclonal Antibody
- 货号：YT4861
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Oxidative phosphorylation;>>Metabolic pathways;>>Lysosome;>>Phagosome;>>mTOR signaling pathway;>>Synaptic vesicle cycle;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Tuberculosis;>>Human papillomavirus infection;>>Rheumatoid arthritis
- 蛋白名称：
- V-type proton ATPase subunit H
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human ATP6V1H. AA range:341-390
- 特异性：
- V-ATPase H Polyclonal Antibody detects endogenous levels of V-ATPase H protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ATP6V1H;CGI-11;V-type proton ATPase subunit H;V-ATPase subunit H;Nef-binding protein 1;NBP1;Protein VMA13 homolog;V-ATPase 50/57 kDa subunits;Vacuolar proton pump subunit H;Vacuolar proton pump subunit SFD
- 背景：
- This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
- 功能：
- function:Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.,similarity:Belongs to the V-ATPase H subunit family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'' and d). Interacts with HIV-1 Nef protein and AP2M1.,tissue specificity:Widely expressed.,
- 细胞定位：
- Cytoplasmic vesicle, clathrin-coated vesicle membrane ; Peripheral membrane protein .
- Western Blot analysis of various cells using V-ATPase H Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using ATP6V1H Antibody. The picture on the right is blocked with the synthesized peptide.
