USP16 Polyclonal Antibody
- 货号：YT4831
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Ubiquitin carboxyl-terminal hydrolase 16
- 免疫原：
- Synthesized peptide derived from the Internal region of human USP16.
- 特异性：
- USP16 Polyclonal Antibody detects endogenous levels of USP16 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- USP16;MSTP039;Ubiquitin carboxyl-terminal hydrolase 16;Deubiquitinating enzyme 16;Ubiquitin thioesterase 16;Ubiquitin-processing protease UBP-M;Ubiquitin-specific-processing protease 16
- 背景：
- This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,disease:A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1.,domain:The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.,function:Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.,PTM:Ph
- 组织表达：
- Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Western Blot analysis of 3T3 cells using USP16 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
