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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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UEV3 Polyclonal Antibody
- 货号:YT4817
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Ubiquitin-conjugating enzyme E2 variant 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human UEVLD. AA range:141-190
- 特异性:
- UEV3 Polyclonal Antibody detects endogenous levels of UEV3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- UEVLD;UEV3;Ubiquitin-conjugating enzyme E2 variant 3;UEV-3;EV and lactate/malate dehydrogenase domain-containing protein
- 背景:
- function:Possible negative regulator of polyubiquitination.,similarity:Contains 1 UEV (ubiquitin E2 variant) domain.,similarity:In the C-terminal section; belongs to the LDH/MDH superfamily.,similarity:In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.,subunit:Homodimer.,tissue specificity:Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.,
- 功能:
- function:Possible negative regulator of polyubiquitination.,similarity:Contains 1 UEV (ubiquitin E2 variant) domain.,similarity:In the C-terminal section; belongs to the LDH/MDH superfamily.,similarity:In the N-terminal section; belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.,subunit:Homodimer.,tissue specificity:Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.,
- 细胞定位:
- extracellular exosome,
- 组织表达:
- Colon, colon carcinoma cell lines, normal cervical epithelium, carcinomas of the uterine cervix and peripheral blood leukocytes.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using UEVLD Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HepG2 cells, using UEVLD Antibody. The lane on the right is blocked with the synthesized peptide.