Trypsin-1 Polyclonal Antibody
- 货号：YT4753
- 应用：WB;ELISA
- 种属：Human;Rat
- 简介：
- >>Neuroactive ligand-receptor interaction;>>Pancreatic secretion;>>Protein digestion and absorption;>>Influenza A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Trypsin-1. AA range:60-109
- 特异性：
- Trypsin-1 Polyclonal Antibody detects endogenous levels of Trypsin-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PRSS1;TRP1;TRY1;TRYP1;Trypsin-1;Beta-trypsin;Cationic trypsinogen;Serine protease 1;Trypsin I
- 背景：
- This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.,caution:Tyr-154 was proposed to be phosphorylated (PubMed:8683601) but it has been shown (PubMed:17087724) to be sulfated instead. Phosphate and sulfate groups are similar in mass and size, and this can lead to erroneous interpretation of the results.,cofactor:Binds 1 calcium ion per subunit.,disease:Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.,function:Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.,mass spectrometry: PubMed:8683601,PTM:Occ
- 细胞定位：
- Secreted, extracellular space.
- 组织表达：
- Brain,Cajal-Retzius cell,Gastric adenocarcinoma,PCR rescued clones,Prostate,
- Western Blot analysis of various cells using Trypsin-1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from 293 cells, using Trypsin-1 antibody.
