Troponin I-C Polyclonal Antibody
- 货号:YT4747
- 应用:WB;IHC;IF;ELISA
- 种属:Mouse;Rat
- 蛋白名称:
- Troponin I cardiac muscle
- 免疫原:
- The antiserum was produced against synthesized peptide derived from mouse TNNI3. AA range:5-54
- 特异性:
- Troponin I-C Polyclonal Antibody detects endogenous levels of Troponin I-C protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TNNI3;TNNC1;Troponin I; cardiac muscle;Cardiac troponin I
- 背景:
- Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
![](/uploadfiles/PIC/yt4747-wb.jpg)
- Western Blot analysis of mouse cells using Troponin I-C Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
![](/uploadfiles/PIC/yt4747_pic291418522.jpg)
- Immunofluorescence analysis of HepG2 cells, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4747_pic291518522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using TNNI3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4747_pic291618522.jpg)
- Western blot analysis of lysates from mouse heart cells, using TNNI3 Antibody. The lane on the right is blocked with the synthesized peptide.