TP53INP1 Polyclonal Antibody
- 货号:YT4705
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Tumor protein p53-inducible nuclear protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TP53INP1. AA range:171-220
- 特异性:
- TP53INP1 Polyclonal Antibody detects endogenous levels of TP53INP1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TP53INP1;P53DINP1;SIP;Tumor protein p53-inducible nuclear protein 1;Stress-induced protein;p53-dependent damage-inducible nuclear protein 1;p53DINP1
- 背景:
- function:In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.,induction:By adriamycin, gamma irradiation and H(2)O(2), in a p53-dependent way. At lower levels by ultraviolet irradiation.,subcellular location:Concentrated in PML/POD/ND10 nuclear bodies.,subunit:Interacts with TP53 and HIPK2.,tissue specificity:Ubiquitously expressed.,
- 功能:
- function:In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis.,induction:By adriamycin, gamma irradiation and H(2)O(2), in a p53-dependent way. At lower levels by ultraviolet irradiation.,subcellular location:Concentrated in PML/POD/ND10 nuclear bodies.,subunit:Interacts with TP53 and HIPK2.,tissue specificity:Ubiquitously expressed.,
- 细胞定位:
- Cytoplasm, cytosol. Nucleus. Nucleus, PML body. Cytoplasmic vesicle, autophagosome. Shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions, and re-localizes to autophagosomes on autophagy activation.
- 组织表达:
- Ubiquitously expressed.
![](/uploadfiles/PIC/yt4705-wb.jpg)
- Western Blot analysis of various cells using TP53INP1 Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt4705_pic569018522.jpg)
- Western blot analysis of lysates from COLO205, HUVEC, and HepG2 cells, using TP53INP1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4705_pic1073518522.jpg)
- Western blot analysis of the lysates from HT-29 cells using TP53INP1 antibody.
![](/uploadfiles/PIC/394biantaoticc.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).