TNF-IP 1 Polyclonal Antibody
- 货号:YT4684
- 应用:WB;IHC;IF;ELISA
- 种属:Human
- 蛋白名称:
- BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TNAP1. AA range:71-120
- 特异性:
- TNF-IP 1 Polyclonal Antibody detects endogenous levels of TNF-IP 1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TNFAIP1;BACURD2;EDP1;BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2;hBACURD2;BTB/POZ domain-containing protein TNFAIP1;Protein B12;Tumor necrosis factor; alpha-induced protein 1, endothelial
- 背景:
- This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008],
- 功能:
- function:Enhances the PCNA-dependent DNA polymerase delta activity.,induction:By TNF-alpha, interleukin-1 beta and lipopolysaccharide (LPS).,similarity:Belongs to the KCTD10/KCTD13/TNFAIP1 family.,similarity:Contains 1 BTB (POZ) domain.,subunit:Interacts with PCNA.,
- 细胞定位:
- Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
- 组织表达:
- Amygdala,Endothelial cell,Epithelium,Lung,
![](/uploadfiles/PIC/yt4684-wb.jpg)
- Western Blot analysis of various cells using TNF-IP 1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
![](/uploadfiles/PIC/yt4684_pic542818522.jpg)
- Western blot analysis of lysates from HUVEC cells, treated with PMA 125ng/ml 30', using TNAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt4684_pic1072518522.jpg)
- Western blot analysis of the lysates from 293 cells using TNAP1 antibody.
![](/uploadfiles/PIC/28-ihctiantaoticn202207.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).