TNF-IP 1 Polyclonal Antibody

    • 货号:YT4684
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human
      • 靶点:
      • TNF-IP 1
      • 基因名称:
      • TNFAIP1
      • 蛋白名称:
      • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
      • Human Gene Id:
      • 7126
      • Human Swiss Prot No:
      • Q13829
      • Mouse Swiss Prot No:
      • O70479
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human TNAP1. AA range:71-120
      • 特异性:
      • TNF-IP 1 Polyclonal Antibody detects endogenous levels of TNF-IP 1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • TNFAIP1;BACURD2;EDP1;BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2;hBACURD2;BTB/POZ domain-containing protein TNFAIP1;Protein B12;Tumor necrosis factor; alpha-induced protein 1, endothelial
      • 实测条带:
      • 34kD
      • 背景:
      • This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008],
      • 功能:
      • function:Enhances the PCNA-dependent DNA polymerase delta activity.,induction:By TNF-alpha, interleukin-1 beta and lipopolysaccharide (LPS).,similarity:Belongs to the KCTD10/KCTD13/TNFAIP1 family.,similarity:Contains 1 BTB (POZ) domain.,subunit:Interacts with PCNA.,
      • 细胞定位:
      • Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
      • 组织表达:
      • Amygdala,Endothelial cell,Epithelium,Lung,
      • 产品图片
      • Western Blot analysis of various cells using TNF-IP 1 Polyclonal Antibody diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
      • Western blot analysis of lysates from HUVEC cells, treated with PMA 125ng/ml 30', using TNAP1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from 293 cells using TNAP1 antibody.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).