TCF-9 Polyclonal Antibody
- 货号：YT4581
- 应用：WB;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- GC-rich sequence DNA-binding factor 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GCF. AA range:141-190
- 特异性：
- TCF-9 Polyclonal Antibody detects endogenous levels of TCF-9 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GCFC2;C2orf3;GCF;TCF9;GC-rich sequence DNA-binding factor 2;GC-rich sequence DNA-binding factor;Transcription factor 9;TCF-9
- 背景：
- The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene. [provided by RefSeq, Jul 2008],
- 功能：
- function:Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.,sequence caution:Contaminating sequence. The N-terminus matches the 2q37.3 region.,similarity:Belongs to the GCF family.,tissue specificity:Widely expressed in tissues and cell lines.,
- 细胞定位：
- Nucleus, nucleoplasm . Nucleus, nucleolus .
- 组织表达：
- Widely expressed in tissues and cell lines.
- Western blot analysis of lysates from RAW264.7 cells, using GCF Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GCF antibody.
