TACC3 Polyclonal Antibody
- 货号:YT4522
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Transforming acidic coiled-coil-containing protein 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TACC3. AA range:789-838
- 特异性:
- TACC3 Polyclonal Antibody detects endogenous levels of TACC3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TACC3;ERIC1;Transforming acidic coiled-coil-containing protein 3;ERIC-1
- 背景:
- This gene encodes a member of the transforming acidic colied-coil protein family. The encoded protein is a motor spindle protein that may play a role in stabilization of the mitotic spindle. This protein may also play a role in growth a differentiation of certain cancer cells. [provided by RefSeq, Nov 2011],
- 功能:
- function:Plays a role in the microtubule-dependent coupling of the nucleus and the centrosome. Involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors (By similarity). May be involved in the control of cell growth and differentiation. May contribute to cancer.,induction:Up-regulated in various cancer cell lines.,similarity:Belongs to the TACC family.,subunit:Interacts with microtubules. Interacts with CCDC100/CEP120. The coiled coil C-terminus region interacts with AH receptor nuclear translocator protein (ARNT) and ARNT2 (By similarity). Interacts with GCN5L2 and PCAF.,
- 细胞定位:
- Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Cytoplasm, cytoskeleton, spindle pole . In complex with CKAP5 localized to microtubule plus-ends in mitosis and interphase. In complex with CKAP5 and clathrin localized to inter-microtubule bridges in mitotic spindles. .
- 组织表达:
- Epithelium,PCR rescued clones,Skin,
- Western Blot analysis of various cells using TACC3 Polyclonal Antibody
- Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:100(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
- Immunofluorescence analysis of A549 cells, using TACC3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using TACC3 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from 293 and HUVEC cells, using TACC3 Antibody. The lane on the right is blocked with the synthesized peptide.