T3JAM Polyclonal Antibody
- 货号:YT4517
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- TRAF3-interacting JNK-activating modulator
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human T3JAM. AA range:251-300
- 特异性:
- T3JAM Polyclonal Antibody detects endogenous levels of T3JAM protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TRAF3IP3;T3JAM;TRAF3-interacting JNK-activating modulator;TRAF3-interacting protein 3
- 背景:
- The gene encodes a protein that mediates cell growth by modulating the c-Jun N-terminal kinase signal transduction pathway. The encoded protein may also interact with a large multi-protein assembly containing the phosphatase 2A catalytic subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
- 功能:
- function:May function as an adapter molecule that regulates TRAF3-mediated JNK activation.,subunit:Binds to the isoleucine zipper of TRAF3 via its coiled-coil domain.,
- 细胞定位:
- Cell membrane . Golgi apparatus membrane ; Single-pass type IV membrane protein . Lysosome membrane . Mitochondrion outer membrane . Accumulates on the mitochondria after virus infection. .
- 组织表达:
- Blood,Lung adenocarcinoma,
- Western Blot analysis of various cells using T3JAM Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using T3JAM Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using T3JAM Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).