T3JAM Polyclonal Antibody

    • 货号:YT4517
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • T3JAM
      • 基因名称:
      • TRAF3IP3
      • 蛋白名称:
      • TRAF3-interacting JNK-activating modulator
      • Human Swiss Prot No:
      • Q9Y228
      • Mouse Swiss Prot No:
      • Q8C0G2
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human T3JAM. AA range:251-300
      • 特异性:
      • T3JAM Polyclonal Antibody detects endogenous levels of T3JAM protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • TRAF3IP3;T3JAM;TRAF3-interacting JNK-activating modulator;TRAF3-interacting protein 3
      • 实测条带:
      • 64kD
      • 背景:
      • The gene encodes a protein that mediates cell growth by modulating the c-Jun N-terminal kinase signal transduction pathway. The encoded protein may also interact with a large multi-protein assembly containing the phosphatase 2A catalytic subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
      • 功能:
      • function:May function as an adapter molecule that regulates TRAF3-mediated JNK activation.,subunit:Binds to the isoleucine zipper of TRAF3 via its coiled-coil domain.,
      • 细胞定位:
      • Cell membrane . Golgi apparatus membrane ; Single-pass type IV membrane protein . Lysosome membrane . Mitochondrion outer membrane . Accumulates on the mitochondria after virus infection. .
      • 组织表达:
      • Blood,Lung adenocarcinoma,
      • 产品图片
      • Western Blot analysis of various cells using T3JAM Polyclonal Antibody
      • Immunofluorescence analysis of HeLa cells, using T3JAM Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from Jurkat cells, using T3JAM Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).