SR-3A Polyclonal Antibody
- 货号：YT4401
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Serotonergic synapse;>>Taste transduction
- 蛋白名称：
- 5-hydroxytryptamine receptor 3A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human 5-HT-3A. AA range:161-210
- 特异性：
- SR-3A Polyclonal Antibody detects endogenous levels of SR-3A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- HTR3A;5HT3R;HTR3;5-hydroxytryptamine receptor 3A;5-HT3-A;5-HT3A;5-hydroxytryptamine receptor 3;5-HT-3;5-HT3R;Serotonin receptor 3A;Serotonin-gated ion channel receptor
- 背景：
- The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
- 功能：
- function:This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.,miscellaneous:The HA-stretch region of HTR3A seems to be responsible for the low conductance of HTR3A homomers compared to that of HTR3A/HTR3B heteromers.,similarity:Belongs to the ligand-gated ionic channel (TC 1.A.9) family.,subunit:Forms pentahomomeric complex as well as pentaheteromeric complex with HTR3B or HTR3C or HTR3D or HTR3E; homomeric complex are functional but exhibit low conductance, decreased agonist and antagonist affinity with modified voltage dependence. Interacts with RIC3.,tissue specificity:Expressed in cer
- 细胞定位：
- Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
- 组织表达：
- Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
- Western Blot analysis of various cells using SR-3A Polyclonal Antibody
- Western blot analysis of lysates from HT-29 cells, using 5-HT-3A Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using 5-HT-2B antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
