Spindlin-1 Polyclonal Antibody

    • 货号:YT4385
    • 应用:WB;IHC;IF;ELISA
    • 种属:Human;Mouse;Rat
      • 靶点:
      • Spindlin-1
      • 基因名称:
      • SPIN1
      • 蛋白名称:
      • Spindlin-1
      • Human Swiss Prot No:
      • Q9Y657
      • Mouse Swiss Prot No:
      • Q61142
      • 免疫原:
      • The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160
      • 特异性:
      • Spindlin-1 Polyclonal Antibody detects endogenous levels of Spindlin-1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Polyclonal, Rabbit,IgG
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • 纯化工艺:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • 浓度:
      • 1 mg/ml
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • SPIN1;OCR;SPIN;Spindlin-1;Ovarian cancer-related protein
      • 实测条带:
      • 30kD
      • 背景:
      • function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family.,
      • 功能:
      • function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family.,
      • 细胞定位:
      • Nucleus . Nucleus, nucleolus .
      • 组织表达:
      • Highly expressed in ovarian cancer tissues.
      • 产品图片
      • Immunofluorescence analysis of Hela cell. 1,Spindlin-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
      • Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Western blot analysis of lysates from HeLa cells, using SPIN1 Antibody. The lane on the right is blocked with the synthesized peptide.
      • Western blot analysis of the lysates from K562 cells using SPIN1 antibody.