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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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SMVT Polyclonal Antibody
- 货号:YT4349
- 应用:WB;ELISA;IHC
- 种属:Human;Rat;Mouse;
- 简介:
- >>Vitamin digestion and absorption
- 蛋白名称:
- Sodium-dependent multivitamin transporter
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SLC5A6. AA range:551-600
- 特异性:
- SMVT Polyclonal Antibody detects endogenous levels of SMVT protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SLC5A6;SMVT;Sodium-dependent multivitamin transporter;Na(+)-dependent multivitamin transporter;Solute carrier family 5 member 6
- 背景:
- function:Transports pantothenate, biotin and lipoate in the presence of sodium.,similarity:Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.,
- 功能:
- function:Transports pantothenate, biotin and lipoate in the presence of sodium.,similarity:Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.,
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein .; Cell membrane ; Multi-pass membrane protein . (Microbial infection) Exposure to E.coli lipopolysaccharides leeds to reduced cell membrane localization. .
- 组织表达:
- Expressed in microvessels of the brain (at protein level) (PubMed:25809983). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas (PubMed:10329687).
- Western blot analysis of lysates from HepG2 and HUVEC cells, using SLC5A6 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using SLC5A6 antibody.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).